Parcours For the Microbes (FORTHEM)

Les enseignements de cette UE sont délivrés à l'Université de Mayence en Allemagne

This module aims to bring all students to a unique experimental and technical level. Here essential basic techniques will be taught and trained to prepare the students to work in a modern microbiological, molecular biological and biotechnological lab. The conceptual design of the course is to teach the methods in logical order “from gene to protein”. In the accompanying lecture, the theoretical background of the techniques are taught, which are then trained and applied in the experimental part of the module. In the accompanying seminar a scientific correlation to the methods is drawn. Programm :

Lectures (21h) 

Introduction to and theoretical background of advanced laboratory techniques. 

Tutorials (10,5h) 

Data analysis. 

Practice (84h) 

Training of basic methods and lab skills in a modern microbiological, molecular biological and biotechnological lab.: 

Design of oligonucleotid primers 

Polymerase chain reaction (PCR) 

Molecular biological cloning strategies  

Genetic manipulation of microorganisms 

Overproduction of proteins, fermentation 

Purification of proteins, chromatography 

SDS-PAGE, Western blot 

Enzymatic reactions 

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Les enseignements de cette UE sont délivrés à l'Université de Mayence en Allemagne

In this module, comprehensive knowledge of biomolecular interactions is imparted. Biomolecular interactions play a central role in almost every cellular process. These can be interactions from protein to protein, but also from protein to DNA, antibody antigen, lipid to protein or protein to low molecular weight substances such as secondary messengers, hormones or drugs. It is therefore of crucial importance in biology, medicine and biotechnology to analyse and quantify these processes. The aim of this module is therefore to give an overview of the different methods used in modern biology to study and quantify various biomolecular interactions. The functioning as well as the application possibilities of the methods will be explained in an eLecture using video clips, animation films and software applications. The learning success can be checked by the students in small quizzes. In an accompanying seminar, in which the students prepare and present a scientific lecture, selected techniques that have been successfully applied in current research are then discussed. Finally, the module contains an exercise in which a selection of techniques are trained, including the high-end technique of surface plasmon resonance spectroscopy. 

Programm :

Lectures (21h) 

Biomolecular interactions of protein/protein; protein-DNA, protein-lower molecular substances; quantification of interactions. 

Tutorials (10,5h) 

Seminar "Biomolecular interactions". 

Practice (84h) 

Laboratory work on biomolecular interactions of protein/protein; protein-DNA, protein-lower molecular substances; quantification of interactions. 

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Les enseignements de cette UE sont délivrés à l'Université de Mayence en Allemagne

In this module, students will acquire the basic knowledge required to study the physiological function of proteins in filamentous fungi. These organisms are manipulated with molecular biological methods, e.g. to generate mutants in which genes have been inactivated. With these "loss-of-function" mutants it should then be possible to conduct further experiments. For example, components of signal recognition and signal transmission can be specifically switched off to investigate whether the organisms are still able to adapt to changing environmental conditions. Such studies are not only of interest for understanding the adaptability and ecology of eukaryotic microorganisms, but also for the investigation of the molecular basis of host-pathogen interactions, host recognition, differentiation, propagation and vitality of fungi. The aim of the module is to give students a profound insight into methods and basics of modern molecular biology and physiology laboratories.  

Besides the lecture, the module includes a practical part in which "loss-of-function" mutants are generated in filamentous fungi for use in physiological studies. Current molecular biological methods are taught and applied. In addition to the exercises, the students will give lectures on current topics of molecular physiology in fungi. 

Programme :

Lectures (21h) 

Physiological function of proteins in filamentous fungi; introduction to methods. 

Tutorials (10,5h) 

Current topics of molecular physiology in fungi. 

Practice (84h) 

Training in molecular manipulation of higher/filamentous fungi; molecular biological methods of the modern microbiological lab, physiological studies. 

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This module provides an in-depth understanding of the adaptation of microorganisms to changing environmental conditions. Microorganisms are rarely in their optimal growth conditions and must therefore constantly adapt. Their circulation in different environments (soil-food-human continuum) requires the implementation of adaptation mechanisms allowing them to resist to sometimes drastic conditions. 

The objective of this module is therefore to detail the adaptation mechanisms available to microorganisms at the molecular and physiological levels, based on examples taken from different fields, with in particular the study of the adaptation of probiotic bacteria to the gastrointestinal tract or the establishment of resistance to antibiotics with the consequences on health The capacity to adapt to the environment will also be studied in the context of bioremediation. 

The teaching will be divided into lectures allowing the students to acquire fundamental notions. Then, the acquired knowledge will be applied to the realization of theoretical and experimental projects, allowing the students to acquire important technical skills. 

Programm :

Lectures (20h) 

Stress response and adaptation (6h). 

Oxidative stress (2h). 

Structural microbiology (2h). 

Adaptation of soil microorganisms to biodegradation and resistance to different xenobiotics (2h). 

Tutorials (8h) 

Analysis of articles (writing and oral presentation). 

Practice (20h) 

Stress response and adaptation studied using microbiological methods and molecular biology. 

Microbial ecology of soil systems disturbed by heavy metals (2h). 

Antibiotics and alternatives (4h). 

Transfer of antibiotic-resistant pathogenic bacteria between animals, the environment and humans (2h). 

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Les enseignements de cette UE sont délivrés à l'Université de Mayence en Allemagne

In this module comprehensive knowledge of the complexity of microbial interactions with relevance to plant and animal health, as well as within microbial communities, is imparted with a focus on (i) control of biofilms and (ii) signalling in interactions between microorganisms and their host (from parasitism to mutualism). The microorganism–microorganism or microorganism–host interactions are essential for microorganisms to establish in a variety of different environments. The aim of this module is therefore to give an overview of the different aspects of microbial interactions as well as methods used in modern biology to study them. The learning success can be checked by the students in small quizzes. In an accompanying seminar, in which the students prepare and present a scientific lecture, selected top ten papers reporting advances in the field are then discussed. Finally, the module contains practical works in which different microbial interactions are addressed. 

Programm :

Lectures (20h) 

Principles of microbial interactions; biofilms and aggregates as persistence strategies of bacteria in environments; Control and promotion of biofilms; Inter-kingdom signalling: communication in the gradient of interactions from mutualism to parasitism; Interactions between microorganisms and their hosts (host-microorganism interaction mechanisms, immune responses); repertoire of techniques used to study microbial interactions. 

 Transversal theme with Module " Microbial diversity and microbial circulation in ecosystems ": Alternative therapies of microbial diseases (phage therapy, antagonistic or probiotic bacteria…). 

Tutorials (8h) 

Analysis of articles (writing and oral presentation). 

Analysis of results obtained during practice (writing and oral presentation). 

Practice (20h) 

Impact of double symbiosis on host. 

Study of bacterial biofilms and their role in bacterial adaptation. 

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The students will acquire practical and theoretical competences in microorganism diversity and phylogeny. They will use current methods available to analyze taxonomic and functional diversity of different groups of microorganisms in the environment, including soil, the human body and food. They will be able to assess the roles of microorganisms in ecosystem function and dysfunction as well as their movement among and within habitats. 

Programm :

Lectures (20h) 

Stress response and adaptation (6h). 

Oxidative stress (2h). 

Structural microbiology (2h). 

Adaptation of soil microorganisms to biodegradation and resistance to different xenobiotics (2h). 

Microbial ecology of soil systems disturbed by heavy metals (2h). 

Antibiotics and alternatives (4h). 

Transfer of antibiotic-resistant pathogenic bacteria between animals, the environment and humans (2h).

Tutorials (8h) 

Analysis of articles (writing and oral presentation). 

Practice (20h) 

Stress response and adaptation studied using microbiological methods and molecular biology. 

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Programm :

Tutorials (15h) 

 -Project management basics; tools to conduct a literature review such as websites to search articles and software to organize a bibliography; writing a. 

-Visits of research institutes, enterprises or technical platforms. 

-Protecting and commercializing an invention: patents, startups. 

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In preparation for the Master's thesis, the work in a research laboratory will provide to the students the opportunity to pursue a research project defined with the supervisors while being trained in independent scientific research. Students will be introduced into the state of the art of the respective research area. They will present their results in a research colloquium.  

 The accompanying seminars will give the opportunity to a broad range of actors from the campuses of Mainz, Dijon and the Forthem alliance to present their field of research and teaching with the aim to facilitate internships and collaboration across the alliance. 

Programm

-4 week-internship in a research laboratory 

-research colloquia 

-research seminars 

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In preparation for the Master's thesis, the work in a research laboratory will provide to the students the opportunity to pursue a research project defined with the supervisors while being trained in independent scientific research. Students will be introduced into the state of the art of the respective research area. They will present their results in a research colloquium. 

 The accompanying seminars will give the opportunity to a broad range of actors from the campuses of Mainz, Dijon and the Forthem alliance to present their field of research and teaching with the aim to facilitate internships and collaboration across the alliance.  

Programm

-4 week-internship in a research laboratory 

-research colloquia 

-research seminars 

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In preparation for the Master's thesis, the work in a research laboratory will provide to the students the opportunity to pursue a research project defined with the supervisors while being trained in independent scientific research. Students will be introduced into the state of the art of the respective research area. They will present their results in a research colloquium.  

 The accompanying seminars will give the opportunity to a broad range of actors from the campuses of Mainz, Dijon and the Forthem alliance to present their field of research and teaching with the aim to facilitate internships and collaboration across the alliance. 

Programm :

-4 week-internship in a research laboratory 

-research colloquia 

-research seminars 

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In preparation for the Master's thesis, the work in a research laboratory will provide to the students the opportunity to pursue a research project defined with the supervisors while being trained in independent scientific research. Students will be introduced into the state of the art of the respective research area. They will present their results in a research colloquium.  

 The accompanying seminars will give the opportunity to a broad range of actors from the campuses of Mainz, Dijon and the Forthem alliance to present their field of research and teaching with the aim to facilitate internships and collaboration across the alliance. 

 Program

-4 week-internship in a research laboratory 

-research colloquia 

-research seminars 

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Possibilité d'effectuer des stages au semestre 3

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stage de 6 mois obligatoire

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